CD45 MultiDot 525 – 100 Tests

$400.00

Product Description

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes.  CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues.  CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains.  CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.  CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell.  CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling.  CD45 is also important in promoting cell survival modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

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Description

Histogram of leukocytes labeled with CD45 MultiDot 525 nm as measured on an LSR-II violet laser excitation (405nm) captured with a 605nm filter.

Product Information

Size

100 Tests

Dilution*

5 μl/million cells

Host/Isotype

Mouse/IgG1

Class

Monoclonal

Type

Antibody

Clone

H130

Immunogen

Human CD45

Conjugate

525 nm MultiDot

Form

Liquid

Purification

Purified

Storage Buffer

Borate buffer, pH 7.4 with 0.5% BSA and 0.1% sodium azide

Storage Conditions

4˚C do not freeze

Reactivity

Human

Tested Application

Flow Cytometry

Batch

Refer to lot on vial

*Suggested working dilution is given as a guide only.

It is recommended the user titrate the product for use in their own

experiment using appropriate negative and positive controls.

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