Products

MagDot 640 Anti-Human CD45

$420.00

SKU: 221345 Category: Tags: , , ,

The MagDot is a dual-purpose reagent, it combines quantum dots and superparamagnetic iron oxide particles within the same nanoparticle. Cells labeled with MagDots targeting specific antigens will be both fluorescent and magnetic. Therefore, after labeling cells of interest, one can look at the feed to determine the percent positive fluorescently via flow cytometry, and then place the labeled cells in a magnetic separator and analyze both the magnetic (for the enrichment) and the nonmagnetic (for the depletion) of targeted cells without the need for further fluorescent labeling.

The HI30 antibody reacts with all isoforms of CD45, a type I transmembrane glycoprotein expressed on the surface of most hematopoietic cells except mature erythrocytes, platelets, and plasma cells; expression of CD45 is lost during differentiation of these cell types. CD45 is a member of the protein tyrosine phosphatase family and functions in a number of immunoregulatory processes, including cell activation, growth, differentiation, and oncogenic transformation. The leukocyte common antigen (LCA), the region recognized by the HI30 antibody, is an extracellular region located proximal to the membrane and common to all isoforms of CD45.

For Research Use Only. Not fur use in diagnostic procedures. Not for resale.

Product Information

Number of tests

20 tests

Dilution*

15-20 µL/million cells

Antibody Concentration

0.08 mg/mL

Host/Isotype

Mouse/IgG1

Particle Concentration

1011 particles/mL

Iron Concentration

0.5 mg/mL

Class

Monoclonal

Antibody per MagDot

700-750 molecules/ MagDot

Type

Antibody

Clone

HI30

Immunogen

Human CD45

Conjugate

640 nm MagDot

Form

Liquid

Purification

Purified

Storage Buffer

Borate, pH 7.4 with 0.5% BSA, 0.1% sodium azide

Storage Conditions

4˚C do not freeze

Reactivity

Human

Tested Application

Flow Cytometry and Magnetic Separation

Catalog Number

MagD-CS-640-H45

 

*Suggested working dilution is given as a guide only. It is recommended the user titrate the product for use in their own experiment using appropriate negative and positive controls.
*Time for separation for the labelled cells will depend on magnetic field strength, gradient used and no of particle bound/ cell.

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