MagDot 640 nm CD45 – 20 Tests

$350.00

The MagDot is a duel-purpose reagent, it combines quantum dots and superparamagnetic iron oxide particles within the same nanoparticle. Cells labeled with MagDots targeting specific antigens will be both fluorescent and magnetic. Therefore, after labeling cells of interest, one can look at the feed to determine the percent positive fluorescently via flow cytometry, and then place the labeled cells in a magnetic separator and analyze both the magnetic (for the enrichment) and the nonmagnetic (for the depletion) of targeted cells without the need for further fluorescent labeling.

These MagDots are conjugated to the CD45 (LCA, leukocyte common antigen) which is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not fur use in diagnostic procedures. Not for resale.

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Description

Product Information

Size

20 tests

Dilution*

10 µL/million cells

Host/Isotype

Mouse/IgG1

Class

Monoclonal

Type

Antibody

Clone

H130

Immunogen

Human CD45

Conjugate

640 nm MagDot

Form

Liquid

Purification

Purified

Storage Buffer

Borate, pH 7.4 with 0.5% BSA and 0.1% sodium azide

Storage Conditions

4˚C do not freeze

Reactivity

Human

Tested Application

Flow Cytometry and Magnetic Separation

Batch

Refer to lot on vial

*Suggested working dilution is given as a guide only.

It is recommended the user titrate the product for use in their own

experiment using appropriate negative and positive controls.

 

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