F.A.Q.’s

Current wavelengths for MultiDots are: 420, 440, 520, 580, 610, 640, 655, 700 and 800. Current wavelengths for MagDots are: 580, 610, 640, and 655.

The particles are best excited at UV and Violet. Please refer to the Data and Images section for more details about each wavelength.

MultiDots: ~ 60 nm; MagDots: ~110 nm SpiDots: ~80 nm (These are average values, the product details in each product has more information)

MultiDots are on average 60 nm and therefore would not be suitable for intracellular staining without cell permeabilization. MagDots are even larger and would be difficult for intracellular labeling. We have had customers have success with both particles using phagocytic cells for the internalization of the particles.

Any buffer that is suitable for use with cells and biological materials is fine to use with CQT reagents, this includes blocking buffers.

CQT reagents do not degrade with normal fixative processes. Our reagents have been fixed using up to 4% paraformaldehyde with no degradation of the fluorescent signal.

We performed a 10-week shelf-life study whereby we left our reagents at room temperature in ambient lighting. There was no loss of signal after this 10-week period. Our reagents are very photostable and temperature stable.

Yes, please refer to the resources section in the product of interest.

For both the SpiDots and MagDots a 15-minute collection time is sufficient in the CoreMag2T magnet. However, if the magnetically labeled population is very rare, collection times of up to 30 minutes is recommended.

The SpiDots would be around the same collection time as other magnetic-only nanoparticles sold with their respective magnets. The MagDots being a dual reagent are only partially magneti. Collection times in weaker open magnetic systems can require an additional 30 minutes for the desired purity and recovery.

All of our magnetic reagents work in packed column systems without affecting recovery. The SpiDots are magnetic only; therefore if an additional fluorescent marker is needed that would need to be added separately. The MagDots are both fluorescent and magnetic thus if the marker is the MagDots label, after magnetic separation there is no need for an additional fluorescent label.

Yes, you can. Refer to the resources section to learn more on fluorophores with similar emission peaks to CQT reagents. To help build a panel we recommend using the design your panel builder on our website. MultiDots and MagDots are excitable by lasers other than UV or violet; therefore, care has to be taken when building your panel so that there is no spillover from other channels.

Yes you can. The narrow emission and unique spectral emission enables multiplexing. Use the design your panel to pick color combinations that prevent emission spillovers.

We absolutely can conjugate antibodies not listed in our current catalog. We are adding new products weekly. Please fill in the form in the Contact page and send us your antibody request. We will procure it from our numerous antibody suppliers, or if you have in-house ship to us and we’ll conjugate and send it back.

We have conjugation kits available. The resources tab in the custom product listing has detailed instructions on how to conjugate your antibody.