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MagDot 640 Anti-Human CD45 (HI30)

$420.00

The MagDot is a dual-purpose reagent, it combines quantum dots and superparamagnetic iron oxide particles within the same nanoparticle. Cells labeled with MagDots targeting specific antigens will be both fluorescent and magnetic. Therefore, after labeling cells of interest, one can look at the feed to determine the percent positive fluorescently via flow cytometry, and then place the labeled cells in a magnetic separator and analyze both the magnetic (for the enrichment) and the nonmagnetic (for the depletion) of targeted cells without the need for further fluorescent labeling. The HI30 antibody conjugated to this MagDot reacts with human CD45, one of the most abundant hematopoietic markers and one that is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others.

Parameter​

Specification​

Product Name​

MagDot 640 Anti-Human CD45 (HI30)​

Optimal Excitation​

355 and 405 nm​

Emission Maximum​

640 nm​

Class​

Monoclonal Antibody​

Host/Isotype​

Mouse/IgG1​

Reactivity​

Human​

Immunogen​

CD45​

Clone​

HI30​

Tested Dilution​

25 μL per million cells​

Antibody concentration​

0.1 μg/μL​

Iron oxide concentration​

1 mg/mL​

Storage Buffer​

Borate, pH 7.4 with 0.5% BSA, 0.1% sodium azide ​

Volume​

500 μL​

Storage Temperature​

4°C​

Flow Cytometry