MultiDots consist of several quantum dots within a patented PEG polymer coating. The pegylated fluorescent nanoparticles have very tight size distribution and the amine functional groups on the surface allows conjugation of proteins to label cells of interest. MultiDots with a tighter emission spectrum than current fluorescent reagents, reduces spectral overlap, increases assay sensitivity and eliminates complex spectral compensation. The 61D3 antibody conjugated to this MultiDot is specific for human CD14, a 53-55 kDa GPI-anchored glycoprotein. CD14 is highly expressed on monocytes and to a lesser extent on interfollicular macrophages, and some dendritic cells.