Yeast Display Antibody Screening in Just Days
Streamline your Yeast Surface Display (YSD) workflows with MagDots, our dual magnetic-fluorescent nanoparticles that enable immediate fluorescent analysis after magnetic separation.
Save time and resources with streamlined, highly-efficient antibody screening workflows
Yeast Surface Display has revolutionized antibody discovery by enabling efficient screening of large libraries while maintaining genotype-phenotype linkage. However, current techniques involved in separation are tedious and require multiple enrichment steps and individual labeling steps after every round.
Our dual fluorescent-magnetic nanoparticles, MagDots, compatible with open or column-based magnetic separation systems, allow rapid and effective antigen-binding clone enrichment by enabling fluorescent analysis immediately after magnetic separation. This results in time and cost savings coupled with highly efficient cell recovery.
Download our case study to learn how MagDots enabled Dr. William Bret Redwine, Head of Custom Protein Resources at the Stowers Institute for Medical Research, to decrease the YSD workflow time from five weeks to just two days.
Key takeaways include:
- Yeast surface display antibody discovery workflows are complex, tedious, time-consuming, and expensive.
- MagDots demonstrate highly efficient target cell recovery, reducing the number of selection rounds needed to obtain high purity target cell suspensions in YSD workflows.
- MagDots provide dual magnetic-fluorescent properties in a single nanoparticle, enabling magnetically-separated cells to go directly into fluorescent analysis, streamlining the workflow.
- By combining magnetic and fluorescent properties in a single nanoparticle, MagDots are a 2-in-1 laboratory powerhouse, reducing hands-on sample manipulation and instrument time, and simplifying workflows.
Discover our Yeast Surface Display products and resources
Reagents for YSD
MagDots with Streptavidin can be used to tag biotinylated epitope.The reagents used in YSD may be purchased here.
Protocol for YSD
Learn how MagDots can be used to screen engineered yeast strain in a one step label process using CQT’s Core 2T Magnet.
Magnet for YSD
CoreMag2T designed with a 2T magnet enables rapid and effective separation of MagDot labelled cell suspensions within minutes.